Features:
Ease of Processing with GESOLGEL®MTC Media
• GESOLGEL®MTC disperses and hydrates easily in either hot or cold deionized water, forming viscous solutions in cold distilled water.
• In the presence of soluble salts, GESOLGEL®MTC can be used to provide high gel strength at low GESOLGEL®MTC concentrations (normally at approximately half the concentration required for agar).
• At high temperatures, the low viscosity of GESOLGEL®MTC solutions facilitates pipetting, pumping, and pouring upon cooling, GESOLGEL®MTC solutions gel quickly and uniformly.
• GESOLGEL®MTC is able to withstand normal autoclaving conditions.
• GESOLGEL®MTC is generally resistant to enzymatic degradation.
• GESOLGEL®MTC itself is chemically inert to most biological growth media additives (additive must be heated to just above GESOLGEL®MTC gel point before incorporation).
Quality of Microbiological Media Prepared with GESOLGEL®MTC
• GESOLGEL®MTC gels have proven to be a suitable growth matrix for a wide variety of microorganisms, including those traditionally cultured on agar plates as well as other species not easily grown on other substances.
• GESOLGEL®MTC gels are exceptionally clear, making them an excellent analytical tool.
• GESOLGEL®MTC gels have essentially the same shelf life as agar gels.
Table 1. Recommend dosage for part of mediums
(Applications ) | (Dosage) |
MS Medium ,1962 | 0.25%~0.5% |
B5 Medium | 0.35%~0.5% |
N6 Medium | 0.25%~0.5% |
Nitch Medium | 0.25%~0.5% |
White Medium | 0.2%~0.3% |
MT Medium | 0.25%~0.5% |
Nutrient Broth Gels | 0.3% + 0.1%Mg2SO4﹒7H2O |
Eosin Methylene Blue Gels | 0.5% + 0.1%Mg2SO4﹒7H2O |
Advantages of GESOLGEL®MTC Compared to Agar
• GESOLGEL® MTC may be used at approximately half the use level of agar
• GESOLGEL®MTC, produced by a tightly-controlled fermentation process, has consistent product quality. GESOLGEL®MTC is unaffected by the vagaries of natural conditions which affect the basic properties of agar.
• GESOLGEL®MTC gels are remarkably clear in comparison to those formed with agar.
• Gels prepared with GESOLGEL®MTC set faster than those made with agar. In microbiological applications this reduces plate preparation time.
• Gels prepared with GESOLGEL®MTC are stable at high temperatures. In microbiological media, this supports incubation required by thermophilic microorganisms.
• GESOLGEL®MTC contains no contaminating matters (e.g., phenolic compounds) as those found in agar that are toxic to certain sensitive organisms.
The comparison of GESOLGEL®MTC and agar agar
Figure 1. MS Medium | Figure 2. B5 Medium |
Figure 3. N6 Medium | Figure 4. White Medium |